New synch protocols tailored for fixed-time A.I. in Bos indicus-influenced cattle

Using hormone-based protocols to synchronize estrus and ovulation in combination with fixed-time A.I. (no heat detection) has become increasingly popular in the commercial beef industry.

Regents Fellow / AgriLife Research Faculty Fellow / Professor of Animal Reproduction Laboratory / Texas A&M AgriLife Research — Lincoln
Professor - Reproductive Physiology / Texas A&M University – Kingsville

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However, protocols developed for use in straight-bred English and Continental breeds have generally not performed well in Brahman-influenced cattle, with pregnancy rates to fixed-time A.I. often ranging from 35 to 40 percent. As a result, few commercial cattlemen in the southern U.S. have routinely used these technologies.

One of the most successful Bos taurus-based protocols, the so-called “Five-Day CO-Synch + CIDR,” employs application of a CIDR device plus an injection of gonadotropin-releasing hormone (GnRH) on day zero. The CIDR is then removed on day five, and a double dose (all at once or split into two injections eight hours apart) of prostaglandin (PG) is injected.

Fixed-time A.I. is at 72 hours in conjunction with a second injection of GnRH (Figure 1A). Pregnancy rates to fixed-time A.I. in Bos taurus and Bos taurus crosses have been reported to range from 55 to 60 percent in mature cows and 50 to 55 percent in heifers.

<a href=Time lines for application of treatments related to the 5-Day CO-Synch" width="610" />

Unfortunately, this protocol does not result in acceptable fixed-time A.I. pregnancy rates in Brahman-influenced cattle. However, modifications of the procedure, developed at the Texas A&M AgriLife Research Station in Beeville and called Bee Synch, yielded excellent results in these cattle types.

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